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In this study, response of twenty Iranian hexaploid wheat (Triticum aestivum L.) genotypes were investigated for anther culture. Donor plants were
grown in a greenhouse using randomized block design with three replications. Anthers were plated on a CHB liquid medium containing 90 g/1 maltose, 0.5
mg/12,4-D and 0.5 mgll kine tine. All genotypes produced calli, embryoids and green plants. Analysis of variance indicated a highly significant difference
between the genotypes for all the traits studied (numebr of the embryoids per 100 anthers; total, albino and green regenerated plants per 100 anthers).
'Moghan l' cultivar produced the highest green and total regenerated plants (16.46% and 29.71 % respectively) and the M- 75-13 genotype showed the
lowest level of green and total regenerated plants (0.85% and 1.99%). 'Atrak' cultivar had the highest value of callus production (114.6%) whereas' Alborz'
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and 'Pastoor' cultivars produced the lowest level (11.1 % and 11.8%
respectively). 'Atrak' and 'Rasool' cultivars produced the highest level of
albino plants. It could be concluded that genotypic response to anther culture and independency of androgenic traits are evident.