Plant growth promoting rhizobacteria (PGPR) specially some strains of Pseudomonas fluorescens produce siderophores, and ionophores that chelate some nutrient elements thereby improving their uptake. Also, these bacteria may produce growth regulator substances which affect availability of nutrient elements. This study was carried out to determine the potential of native P. fluorescens strains to improve nutrient uptake by wheat. For this purpose, 40 strains of P. fluorescens were used to inoculate the spring wheat, Moghan 1 variety, in a RCBD experiment with four replications, under greenhouse conditions. The data were analyzed using MSTATC program. Based on results, inoculation with different strains improved the nutrient uptake by wheat, but increase was significant (P<0.01) only in Fe and Mn content (P<0.05) in inoculated plants. The B9 and B36 Strains presented the upper concentration and amount of Fe uptake with their differences with control being significant (P<0.05). Biological yield presented the highest significant correlation with Fe — uptake (R20.9 ) and lowest for K-uptake (R2=0.22 ).