This study was done in order to determine the cellulolytic-bacterial microtlora of silkworm (Bombyx mori) gut. A facultative anaerobic and cellulolytic mesophilic bacterium determined as Cellulomonas sp. (sw971)was
isolated from silkworm gut at 25° c and 30° c in cellulose agar. The strain generation time was almost 3 hours. A maximum level of endoglucanase (E. C.
3. 2. 1. 91) was observed 6 days after culture. Thus the gut of silkworm is an ecological niche for Cellulomonas sp. that can have a potential usage in biotechnology