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Abstract

This research was carried out to evaluate Iranian Hordeum b\1lbosum genotypes for haploid production in barley in comparison with PBl gGnotype (as control). F3 plants from crosses between the spring barley cUltivars Trompilo , c.c.89 (23216) and c.c. 89, Eneldo"s" (23176) were used as female parents and four H. bulbosum genotypes: 1180,76063, TN-2-173 and PBl were used as male parents. During the experiment the following four parameters were studied. These include percentage of seed setting, embryos excised, embryos germinated and plantlets produced. Comparisons among the bulbosum genotypes and the barley genotypes for above parameters were made using chi-square test. Cytological studies showed that the bulblosum
genotypes 1180, 76063 and TN-2-173 were all tetraploid (2n=4x=28). The progenies of interspecific hybridization between diploid Hordeum vulgare and tetraploid H. bulbosum were all triploid (3x=21) while the progenies of interspecific hybridization between diploid H. vulgare and diploid H. bulbosum were haploids (n=x=7). Statistical studies showed that tetraploid H. bulbosum genotypes 1180, 76063 and TN-2-173 differed significantly in seed setting (%)
in crosses with barley. There was not any significant difference between the two barley genotypes 23216 and 23176 in crosses with tetraploid bulbosum but there was significant difference between the barley genotypes in seed setting (%) and embryos excised (%) in crosses with diploid H. bulbosum genotype PBI. Therefore it is concluded that the studied Iranian H. bulbosum genotypes are not suitable for haploid production in barley. Meanwhile, H. bulbosum genotype PBI can be successful1y used for barley haploid breeding
programs in our country.

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