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This study was conducted to determine the genotypic effect on callus induction in barley using 4 two-row and 4 six-row Japanese and Iranian barley
selected from cereal collection of the Department of Agronomy, College of Agriculture, University of Tehran. Two weeks after pollination, immature embryos were dissected and planted in petri dishes using a solid MS medium. The petri dishes were incubated at 25 + 1 C_ The induced calli were sub-cultured on the same medium after about one month. In this study, the
following variables were analyzed: 1) number of responding embryos; 2) growth rate of calli and 3) size of calli. The analysis of variance indicated a significant effect of genotype on the variables. The number of responding
embryos was highest for Zarjo (a six-row genotype) however, most of its calli were nonembryogenic. Barley No. 17( a two-row genotype) from the collection showed the highest size of calli most of which were embryogenic. While there
was no significant effect of spike per plant, a significant effect was observed on plants within genotypes. Significant differences were observed among planting dates for aU of the variables. A highly significant interaction was also observed between two sub-cultures.