Eighteen wheat genotypes (Triticum aestivum L. 2n=6x=42) as female parents with eleven maize genotypes (Zea mayes L. 2n=2x=20) as male parents (pollen donor) were crossed in order to examine the production of haploid wheat by using, five solid culture media. Wheat and maize genotypes were grown in greenhouse and field and maize genotypes were grown in green house under l&.25 temperature and in the field.The effect of environmental factors on the growth and development of pollen tube of maize, the effect of supplemental materials such as yeast and malt extract concentrations on embryo culture and the efficiency of haploid plant production were examined in this study. The effects of wheat cultivars and maize genotypes on embryo formation and haploid seeds in wheat cultivars indicate that the crossability of wheat cultivars with regard to maize genotypes used are different. Among eleven genotypes of maize used, KSC 647 and KSC MA genotypes did not show any compatibility with wheat but the compatibility of the other genotypes in terms of seed set ranged from 11.11 to 100% in wheat cultivars. The obtained embryo frequency varied from 5 to 86/67% . The results of the effect of environmental factors on the growth and development of pollen tube of maize showed that the use of pollen of maize in 25°c temperature and the stigma feathery in wheat at the time of poffination are very important in production of haploid wheat. Also, the effect of supplemental materials such as malt and yeast extracts on embryo culture showed that culture medium of Y2 MS + malt extract 100 mg/I compared to other used media has increased normal haploid plant formation. The effect of radiation treatments on the growth of pollen tubes indicates that overall effects of green, red, blue, yellow and dark radiation compared to white radiation (standard) were not significant.
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