200 barley land race populations belong to different regions of Iran were assayed electrophoretically (HSGE) for genetic diversity and polymorphism in 5 enzymatic loci. In this study one isozymic locus was found to be monomorph but a large variation was obseved for the four loci(Est 1,Est2,Est3,Est4) .In order to classify 200 land race populations and to study the relationships among them, electrophoresis was carried out on random mixed samples (6-7 plumuic from each population). Genetic distance cocfficients,1-F,were calculared for the observed bands for each sample. Cluster analysis and dendrogram constraction was carried out for 200 landrace populations and 45 origins. 13 clusters were obtained for 45 origins. 26 populations among 200 populations wich showed more diversity were selected to measure alleic frequencies of four loci. From a total of 19 allozymes, 5,6 and 5 alleces were observed at the Estl,Est2,Est3 and Est4 loci , respectively. Nei’s genetic distances and genetic identities worked out for the populations. Cluster analysis was carried out and dendrogram was generated from genetic identities. 9 clusters were obtained for 26 populations . Nei’s genetic heterozygosity(h), mean heterozygosity in all loci(II) , and proportion of polymorphic loci worked out for the populations.
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