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Addition lines of the combination B.napus X B.nigra with added chromosomes of the B.nigra were obtained. A number of lines which were derived from addition lines but with 2n=38 chromosomes showed 3 characteristics from B.nigra (B-genome). The objective of this study was to find B-genome specific RAPD markers in these lines .A total of 4.6 individual plants including 13 different lines and 2 separate bulked DNA from 5 plants of each parent ,B.napus and B.nigra were tesed with twenty lO-mer primers which were B. nigra specific markers . Leaf DNA extracted using a micropreparation method. Concentration of the DNA were determined with Beckman DU-6 spectrophotometer. 25 uIreaction for RAPD were used . Only one primer (opA-19) showed B-genome specific marker in 8 individual plants from 3 lines (825-1,825-3, 825-4, 825-5, 827-2,828-3,828-4, and 828-5). The size of the marker was 1.3 kb. The marker showed consistency with repeatted reaction