Gliadin is one of the storage protein in wheat endosperm which shows high polymorphism .It is controled by a group of genes located on the short arms of homologous chromosomes of group I and 6 About 50 Iranian and exotic wheat cultivars were studied for their Gliadian polymorphism .Using A-PAGE electrophoresis , Marquis was used as check variety. A high degree of polymorphism was found in cultivars under study, especially in gama and omega zones protein subunits. The degree of stainbility of banding were different . The highest density was observed in area between 40 to 60 relative mobility (RM) units. Cultivar grouping was done by UPGMA cluster analysis, and nine cluster were identified. Cultivar such as Arvand , Arvand -1 and Arvand -Mutant , with similar permplasm were located in samç cluster also cultivars like Benzostaya and Ghafghaz with the same Geographical origin were also gouped in same cluster.