In this study, two gram - negative bacteria were isolated on selective medium Si from chickpea rhizosphere. The bacteria had the ability to suppress the Pythium ultimum and promote the plant growth of chickpea. Strain fl(a fluorescent pseudomond) was significantly effective than the control on chickpea grow. Both strains had considerable effect on root development. Also, strain II had better on fresh weight of chickpea as compared to strain I.Strain I produced a kind of antibiotic on NAG (glucose 2%) that could inhibit the casual agent of seed rot and preemmergence of chickpea in the absence of bacterium. Under the same condition, strain II could not produce any antibiotic. Strainli produced siderophore on NAG (glucose 5%) amended with different concentration of FeCI3 and inhibited growth of Geotrichum sp., whereas strain I could not inhibit the growth of the fungus.