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To identify DNA polymorphisms in Sunn pest populations in different regions of IRAN, non parasitized adult females were collected from Fars,
Esfahan, Jiroft, Karadj, Varamin, Mashhad, Boroojerd, Kermanshah and Moghan. DNA was extracted by miniprep method. In this study twenty
primers were selected at random and tested on each sample. For analysis of data primers: UBI, UB2, UB58 and UB62 were selected. These primers amplified template DNA satisfactorily. Grouping was done based on Jaccard's similarity coefficient. These analyses indicated that populations of Karadj,
Varamin, Esfahan and Fars stand in one group and populations of Boroojerd and Kermanshah are in another group, while populations of Moghan, Jiroft and Mashhad go to separate groups. The most genetical differences could be
identified in populatIons of Kermanshah and Boroojerd versus population of Jiroft and the least genetical differences could be identified between Fars and
Esfahan populations.