Using amplified fragment lengh polymorphism (AFLP) in a small gel, generated 171 polymorphic AFLP fragment segregating 99 recombinant inbred lines (RILs) of barley cultivars “Azumamugi” × “Kanto Nakate Gold”. Most of these fragments showed Mendelian segregation of 1: 1 ratio, all being assigned to seven barley chromosomes, indicating that these fragments are reliable as molecular markers for barley genome mapping. Linkage maps were constructed with 173 markers (125 detected loci), consisting of 171 AFLPs and two morphological markers, which covered 875cM.
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