Given numerous molecular marker advantages, at present, marker–based genotyping has been extensively adoped by plant breeders. In this study, using DAF and RAPD technology, relationships of several soybean cultivars were evaluated through simple matching Coefficients. Similarity of cultivars in DAF method (0.704-0.899) was higher than in RAPD (0.561-0.854). Cultivars were better classified by both DAF and combined data (DAF and RAPD), so that Iranian cultivars of the highest relationship occupied the same group. DAF technology allowed studying of soybean genomic DNA with greater resolution, more informative content and free from inherent disadvantages in RAPD.
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