Application of haploid plants in breeding programs can provide a considerable saving of time. This is possible by development of pure doubled haploid lines from haploid plants through doubling of their chromosome complement. In order to produce haploid plants from Indian hexaploid wheats through crossing with maize and also to study genotypic effects of wheat plants, five F1 hybrids of wheat (W1-W5) which had been obtained from crossing of different hexaploid wheat varieties were crossed to a composite variety of maize named Kanchan. In these crosses maize plants were used as pollen parent. After pollination of emasculated wheat florets with maize pollen, for three days hormonal treatment was applied on crossed spikes. Through this operation 75 ppm solution of 2,4-D for two days and 300 ppm solution of Gibberellic acid in the 3rd day after pollination were sprayed on pollinated spikes. Fourteen days after pollination spikes were collected and searched for seed and embryo formation. Response of these wheat genotypes was different for embryo formation rate. The best genotype was W1 with 12.8% embryo formation while the poorest genotype W2 produced 2.5% embryo from total number of obtained seeds. The mean performances of wheat genotypes, W2, W4 and W5 for embryo production rate were significantly lower than W1 and W3. The pollen tube growth measurements conducted on W1 and W5 crosses showed a significant difference in length of pollen tube, 30 minutes after pollination inside the stigma of these two wheat parents. W1 possessed a higher mean of pollen tube length than W5, which was in agreement with their embryo production percentage. It was concluded that the lower speed growth of maize pollen tube inside the stigma of some wheat genotypes, followed by lower performance in embryo production may be due to some weak systems of crossability, different from Kr genes system which need to be further studied.
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