Glyphosate (common name Randap) is one of the most effective general herbicides becoming widely recognized nowadys. This chemical cannot be used to control weeds in soybean fields, because the crop plant is not adequately resistant to the herbicide, therefore, in order to be able to use it, resistance of soybean to the herbicide must somehow be enhanced. Pot and hydroponic experiments were carried out to study trait diversity in a community of soybeans. Two experiments performed were as follows: I) Two cultivars of soybean (L11 and Williams) in pot and hydroponic media, along with different doses of Glyphosate application were employed to identify the suitable dosage for screening soybean cultivars. II) The resulting and selected doses in experiment I were used to investigate the diversity of resistance of soybean varieties to the herbicide and to subsequently pick up the resistant cultivars. Results of the first experiment indicated that the best dosages for suitable screening of soybean cultivars (in the pot experiment) were 300 and 400 mgai/L and in the hydrponic experiment it was 0/3 mgai/L of glyphosate. Results of the second experiment indicated that there is a correlation of 0/89 between pot and hydroponic experiments and there are high correlation observed among measured parameters in any one of these two screening systems. Even though both screening systems indicated significant differences among soybean cultivars with respect to tolerance to glyphosate, but this is not enough for breeding of soybean with significant resistance to the herbicide. For production of resistant cultivars it is recommended to use new breeding methods such as recombinant DNA technology followed by subsequent transfer of the resistant gene to the plant.