Four hundered isolates of Pseudomonas were isolated from rhizosphere of wheat, their antagonistic activities against teliospore germination of Tilletia laevis race L10 being determined. Seventeen representative isolates were selected for further investigation. Production of antifungal metabolites, volatile compounds and sidrophore by bacterial isolates were the investigated isolates. Slected bacterial isolates based on the biochemical, physiological, nutritional characteristics and also based on a comparison of electrophoretic profiles of cell protein, were identified. Using phenotypic features determination and protein electrophoretic pattern were compared cell free. Metabolites of Pseudomonas fluorescens bioI B9, P.fluorescens bioIII C21, P.fluorescens bioV D23, and volatile compounds of P.fluorescens bioIII D22, P.fluorescens bioI B9, decreased teliospore germination. Sidrophore of P.putida bioA E16 and P.fluorescens on king’s B medium inhibited growth of Geotrichum candidum. In greenhouse experiments, P.fluorescens bioIII D22 reduced common bunt significantly (P<0.05). In all cases skimmed milk formulations were more effective than application of bacterial cell devoid of this additive.
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