The response of six Iranian onion cultivars “Ghermez-e-Azarshahr”, “Sefid-e-Kashan”, “Sefid-e-Qom”, “Dorcheh-e-Isfahan”, “Gholigheseh-e-Zanjan”, “Tarom-e-Olya” as well as the effect of sucrose, maltose and glucose on in vitro gynogenesis of these cultivars were investigated. The most responsive cultivar was “Sefid-e-Kashan” (3.34% of gynogenic embryo), while the least responsive one was “Dorcheh-e-Isfahan” (0.07 % of gynogenic embryo). Difference as to rate of embryo and plant regeneration among different culture media were highly significant. Embryo rate in F6S medium (containing sucrose) was three times that of in F6M (containing maltose). Chromosome count in meristem of root tips showed that 70% of regenerated plants were haploids (n—8).
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