Thirty-two multiparous cows were employed in a randomized complete block design to evaluate the effects of Kilka meal on production and composition of milk in Holstein cows. Experimental diets (1-4) with respectively 0, 1, 2 and 3 percent kilka meal included 44.4% alfalfa hay, and 55.6% cottonseed containing concentrate. Diets were isonitrogenous and isocaloric. Milk yields were 28.94, 31.31, 30.16 and 32.42 Kg/d for diets 1 to 4, respectively. 4% FCM yields were 22.45, 24.83, 24.3 and 24.83 Kg/d for diets 1 to 4 respectively. 3.5% FCM yields were 24.69, 26.63, 26.03, 26.83 Kg/d which were greater than that in control (P<0.01). Percentage and content of fat were not affected by diets. Dry matter intake for milk yield (kg/d) was 0.69, 0.64, 0.68, and 0.63 respectively. Milk protein of 3.35, 3.32, 3.39, 3.48% as well as Lactose percentage of 4.94, 4.79, 4.71, and 4.83 were recordable for diets 1-4 respectively. Percent SNF and total solids were not influenced by diets (P>0.05). Plasma urea nitrogen was greater (P<0.01) for control diets. Significant increases in plasma total protein and glucose were indicative of Kilka meal utilization as compared with control (P<0.01). There were no differences observed for fecal pH. However, fishmeal supplementation substantially improved apparent organic matter as well as crude protein digestibility but it decreased crude fat digestibility as relative to control (P<0.01).
)