Different callus — induction, and regeneration media were used for varieties Nemat, Neda, and Khazar Highest callus -induction and regeneration frequency was obtained for variety Nemat. Nemat as a commercial rice variety in Iran was transformed through biolistic approach. Embryogenic portion of calli derived from mature seeds were bombarded with gold particles coated with f3- glucuronidase gene under the control of Acti promoter. Analysis of transformed calli for the presence of f3- glucoronidase activity was conducted in calli, using staining solution for Gus assay. After optimization of this method for rice by use f3- glucoronidase, embryogenic calli derived from mature seeds of variety Nemat were bombarded with gold particles coated with barley chitinase, and hpt genes under the control of Acti, and CaMV35S promoters, respectively. The bombarded calli were selected on callus — induction media supplemented with 50 mg/l hygromycin B. Resistant calli were regenerated on regeneration media containing hygromycin B. PCR analysis of Putative transgenic plants derived from variety Nemat showed the integration of at least one copy of hpt gene into the rice genome.
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