Karyotypic studies were carried out on twenty hull-less barley (Hordeum vulgare L.) genotypes using squash technique and 2% (w/v) aceto-carmin staining method. Chromosomal parameters examined were as follows: long arm (L), short arm (S), total chromosome length (TL), arm ratio (AR), r-value (S/L), form percentage of chromosome (%F), total form percentage of karyotype (%TF), total chromatin length (X) and the number of satellites. The resultant data were tested for normality and then were analyzed according to two-factorial experiments on the basis of completely randomized design (CRD) with 5 replicates of cells. The first factor was considered as genotypes with 20 levels and the second was considered as chromosomes with 7 levels. ANOVA indicated marked between-genotypes differences for L, S and TL, and conspicuous between-chromosomes differences for whole characters determined. Means comparisons were carried out using Duncan’s multiple range test. All genotypes examined were diploid. The largest chromosome and the most chromatin length were detected in G13 while G4 demonstrated the smallest and the least, correspondingly. All karyotypes were determined in 1A class of Stebbin’s classification and all seven chromosomes were identified as medium region (m) of Levan’s chromosome nomencluture.
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