Microsatellite markers were employed for germplasm analysis and an estimation of the genetic relationship and diversity among 21 genotypes Iranian bread wheat. Thirty-five microsatellite markers were used in the analysis. A total of 199 alleles were detected. The number of alleles per microsatellite marker varied from 2 to 10 with an average of 5.37. The averages for polymorphism information content and genetic similarly were 0.56 and 0.328 respectively. The highest level of genetic similarity (0.755) was found between Ghods and Alvand genotypes while the lowest (0.188) between Bolani and Niknejhad. The results indicated that the genotypes could be distinguished and clustered into 4 groups through UPGMA clustering method. The data suggest that a relatively small number of microsatellites can be used to estimate genetic diversity in the germplasm of Triticum aestivum. This also confirms the putative potential of microsatellite marker for the analysis of germplasm in wheat.
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