The proportion of its fatty acids determines the quality of oilseed rape. In the present study RAPD, ISSR and SSR DNA markers were used to construct a genetic map of a doubled haploid population with 119 individuals in oilseed rape. A total of 151 markers were assigned into 21 linkage groups and the total genome length corresponded to 1173 cM. QTL analysis for five fatty acids including palmitic, stearic, oleic, linoleic and linolenic acids identified 13 QTLs. Three QTLs were mapped for palmitic acid and explained about 31% of phenotypic variation. For stearic acid, three QTLs were detected that explained 30% of phenotypic variation. Two QTLs were identified for oleic acid; a major one explained 20.2% of phenotypic variation. For linoleic acid, two QTLs were mapped; the major one in linkage group one explained 48.5% of the phenotypic variation. Five QTLs were identified for linolenic acid; one QTL with oleic acid and one with linoleic acid were similar ones. QTLs identified in this research can be used for mapping the important genes in fatty acid composition. The congregations of markers with these QTLs provide an opportunity for marker- assisted selection in oilseed rape breeding programs.
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