During the years 1997-1998, thirty-one Agrobacterium isolates were obtained from gall and sap of infected grapes as well as from soil in vineyards of Fars and Kohkilouyeh and Boyerahmad province. Two pathogenic and one non- pathogenic isolates of Agrobacterium tumefaciens Biovar1 were selected and their cell protein patterns compared through gel electrophoresis. A pathogenic and one non- pathogenic isolate of A. vitis and A. tumefaciens Biovar1 were selected from which antisera were prepared. Serological studies based on agar gel diffusion placed bacterial isolates in 4 serogroups while slid agglutination test placed the isolates in 6 sergroups. A comparison of cell proteins pattern showed that A. vitis and A. tumefaciens Biovar1 isolates were heterogenous, even though similar protein bands existed among the isolates.