Chelcheragh lily is a wild attractive species that grows naturally in Damash area in Ammarloo region, Guilan province, Iran. Asiatic hybrids, as commercial lilies, have been resulted from different Asian species. For establishment and mass production of disease free bulblets during a short period of time, experiments were conducted in a factorial arrangement of explant sites (scale base and apex), combination of BA and NAA, each at three levels (0, 0.01, 0.1 mg/l) in a completely randomized design with three replications. Hypoclorite, ethanol and hot water treatments were employed for disinfection. To decontaminate explants 53?C and 55?C hot water was employed for Chelcheragh and Asiatic hybrid respectively. The highest number of bulblets as well as the highest weight of bulblets were obtained from Chelcheragh lily while the Asiatic hybrid explants produced a lot of roots. Chelcheragh lily produced the highest number of bulblets on MS basel medium with no growth regulators and MS supplemented with 0.01 mg/l NAA. The highest weight of bulblets were obtained on medium supplemented with 0.01 mg/l NAA. Asiatic hybrid showed the highest number and weight of bulblets on MS supplemented with 0.1 mg/l NAA and 0.1 mg/l BA. Both lilies emerged with the highest number of roots on MS medium containing 0.1 mg/l NAA. Effects of bulblet sites were not significant on number of bulblets, but the highest weight of bulblets and a large number of roots were obtained from base segments of scales. Developmental patterns of lilies were not similar. The explants of Chelcheragh produced large bulblets without any leaves whereas, Asiatic hybrid emerged with leafy scales.
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