Bacterial canker disease is prevalent in all stone fruit growing areas and mainly on cherry trees. As susceptibility of sweet cherry to the disease is dependent on tree species, using resistant cultivars will decrease the disease occureence rate. In this study 21 sweet cherry cultivars grafted on Pronus mahaleb were tested for determination of their partial resistance to Pseudomonas syringae under greenhouse as well as field conditions. The bacterial agent was injected into cherry seedling and rate of disease progress measured. Results indicated significant differences among the tested cherry cultivars. Cherry cultivars Shoaosaltaneh, SiaheMashhad, Blamarkad, Shabestar, Hajuosefi, Napelon, DirraseItalia, RoodChoon and Ghazvin exhibited more resistance. Cherry cultivars Hybrid 1 Karadj, SiaheDaneshkadeh, Hamedan, Lambert, GhermezeRezaieh, Mashhad, ZardeDaneshkadeh, Arak, Abrdeh, and Mikers were categorized as semi-resistant. BloddyBarbon and GhermezeBagheno were categorized as susceptible. None of the tested sweet cherry cultivars showed complete resistance to bacterial canker disease.